Stem cell exhaustion, regeneration, and rejuvenation
Aging of hematopoietic stem and progenitor cells (HSPCs) impairs regenerative capacity and predisposes to hematological diseases. Here, we constructed a comprehensive single-cell transcriptomic atlas comprising 186,123 CD34+ HSPCs spanning early prenatal development (6 post-conception weeks) …
Partial reprogramming with Yamanaka factors effectively reverses cellular aging but poses potential oncogenic risks, limiting clinical translation. To identify safer rejuvenation induction factors (RIFs), we integrated five aging datasets and compared them with single-cell transcriptomics …
Cellular senescence is a key driver of kidney aging, leading to functional decline and increased susceptibility to chronic kidney disease. While the senolytic combination of dasatinib and quercetin (D + Q) has shown promise in …
This review examines how polyamines (especially spermidine) regulate autophagy—the cell's cleanup system—in skeletal muscle, and how exercise amplifies this process to combat age-related muscle loss. The authors propose that spermine oxidase, an enzyme that produces …
This review synthesizes evidence that natural killer (NK) cells—a type of immune cell—undergo age-related changes that impair their ability to clear damaged cells and regulate inflammation, contributing to aging-related diseases. The authors propose NK cell …
Aging remodels compartmentalized nicotinamide adenine dinucleotide (NAD⁺) circuits in ways that influence stress responses, senescence, tissue repair and susceptibility to fibrosis. Beyond its classical role as a redox cofactor, NAD⁺ fuels sirtuins, PARPs and CD38, …
This review examines how immunosenescence (age-related immune decline) drives lung diseases like COPD, fibrosis, and cancer, and surveys emerging treatments including senolytics, stem cell therapy, and lifestyle interventions. While it synthesizes current knowledge well, it's …
Progressive aging of bone marrow hematopoietic stem cells (HSCs) underlies clonal hematopoiesis and age-associated hematologic disorders. Defining early molecular events driving HSC functional decline is essential for rejuvenation strategies. Here, we identify P-selectin (Selp) as …
A decline in Klotho expression is a defining feature of aging and contributes to cellular dysfunctions. Here, we developed an engineered IVT Klotho mRNA incorporating ARCA capping. Ψ-modification and poly(A) tailing, delivered using a hyperbranched …
This review challenges the assumption that senescent cells are always harmful, showing that some actually support healthy aging through wound healing and tissue maintenance. The authors propose a new strategy: prevent bad senescence while selectively …
The bone marrow niche (BMN) plays a central role in regulating hematopoietic stem-cell (HSC) maintenance, lineage commitment, and immune homeostasis, while also supporting osteogenesis and maintaining skeletal integrity. Once considered static, the BMN is now …
INTRODUCTION: Autologous biofillers are emerging blood-derived regenerative treatments for soft tissue augmentation and regeneration. Despite increasing use, there is limited consensus regarding their definition, preparation, mechanisms, indications, efficacy, longevity, and safety. AIMS: To systematically review …
Glioblastoma is a highly aggressive primary brain tumour marked by extensive genomic and epigenomic alterations, cellular heterogeneity, and therapeutic resistance. Despite maximal surgical resection followed by chemoradiotherapy, median survival remains approximately 15 months, reflecting the …
Given the involvement of long non-coding RNA-differentiation antagonizing non-protein coding RNA (lncRNA DANCR) in osteoporosis (OP), this study aimed to investigate its role in osteogenic differentiation (OD) of bone marrow mesenchymal stem cells (BMSCs) and …
Sarcopenia, the progressive loss of skeletal muscle mass and function with age, is a major contributor to frailty and decreased quality of life in older adults. While physical exercise remains the most effective intervention, its …
This review examines 'partial reprogramming'—a technique that temporarily activates rejuvenation factors to reverse aging hallmarks in cells and tissues without turning them into cancer-prone stem cells. Early evidence suggests it can restore tissue function and …
Scientists developed a technology that uses electromagnetic fields to remotely activate specific genes in living mice with precise timing and location control. They tested it by partially reversing aging processes, modeling Alzheimer's disease, and treating …
Exosomes-small extracellular vesicles containing protein, lipid, and nucleic-acid cargo-modulate key pathways involved in inflammation, angiogenesis, and extracellular matrix remodeling. This narrative review synthesizes current pre-clinical and clinical evidence supporting exosome-based interventions in cutaneous wound healing …
Researchers created a gel containing special vesicles from stem cells that reduces inflammation and cellular aging in aging bone, significantly improving bone and tendon healing in an osteoporosis model. This demonstrates a novel 'senomorphic' approach—directly …
Astrocyte senescence (astrosenescence) has emerged as a potential mechanism through which ageing may progressively impair glial homeostatic neuronal support and render the brain more vulnerable to neurodegenerative diseases such as Parkinsons disease (PD). Yet human …