Whole-genome studies of aging, GWAS, and longevity-associated variants
Glioblastoma is a highly aggressive primary brain tumour marked by extensive genomic and epigenomic alterations, cellular heterogeneity, and therapeutic resistance. Despite maximal surgical resection followed by chemoradiotherapy, median survival remains approximately 15 months, reflecting the …
The CRISPR/Cas9 method facilitates targeted disruption of gene sequences, providing a reliable means to analyze gene-dependent regulatory pathways. This study aims to investigate melanogenesis in p21-knockout B16F1 cells generated by the CRISPR/Cas9 system. The mutation …
Lysosomal dysfunction is a prominent feature of neurodegeneration and aging, yet how primary defects in lysosomal trafficking are converted into progressive cellular decline remains poorly understood. Niemann Pick disease type C (NPC), caused by impaired …
AIMS: Numerous preclinical studies suggested that targeted inhibition of mammalian target of rapamycin (mTOR) may be beneficial for the treatment of osteoporosis. However, the relevance of these findings to human populations remains unclear. We hypothesized …
Intermittent fasting (IF) ameliorates metabolic dysfunction-associated steatotic liver disease (MASLD), but the underlying mechanism remains unclear. Combined CUT&Run and transcriptomic analysis shows that hepatic Ahcy is controlled by the super‑enhancer (SE) and acts as a …
Human longevity is a complex trait shaped by genetic background and population-specific factors. Calabria, a region in Southern Italy with a high prevalence of centenarians and relative genetic isolation, is a valuable model for investigating …
Aging is characterized by progressive oxidative stress, mitochondrial dysfunction, chronic inflammation and intestinal barrier impairment, contributing to increased susceptibility to age-associated disorders. Targeting redox imbalance and epithelial dysfunction represents a promising therapeutic strategy to promote …
CTC1-STN1-TEN1 (CST) is a heterotrimeric, RPA-like complex that binds single-stranded DNA, stimulates DNA polymerase α-primase, and functions in several genome maintenance pathways, including telomere maintenance and DNA replication and repair. During telomere replication, CST prevents …
Age is the dominant risk factor for cardiovascular disease, yet individuals of the same chronological age can differ markedly in the organs and biological pathways through which cardiovascular vulnerability emerges. We used deep learning to …
Experimental evolution studies with Drosophila melanogaster have long played a role in the effort to dissect the genetic basis of aging and longevity. While selection for postponed reproduction reliably extends lifespan, additional phenotypic consequences and …
The APOE gene is a critical determinant of human healthspan and longevity, with the rare{varepsilon} 2 allele traditionally viewed as a universal protective factor against Alzheimer s disease (AD) and a driver of exceptional lifespan. …
R-loops are three-stranded structures consisting of a DNA:RNA hybrid and a single-stranded DNA, which play essential biological roles in eukaryotes. However, their functions during hybridization and the long-term evolutionary trajectory of polyploidization remain unclear. Here, …
Aging is the primary risk factor for most chronic diseases, and the global rise in aging populations presents an escalating challenge due to functional decline and multimorbidity. Gene therapy provides a versatile framework for modulating …
Alternative lengthening of telomeres (ALT) is a specialized telomere extension mechanism associated with 5-10% of all cancers1. Although ALT has been linked to epigenetic dysregulation and genome instability, specific genomic and epigenetic rearrangements generated after …
BACKGROUND: Chronic psychological stress is a well-recognized factor in the exacerbation of allergic diseases, with IgE playing a central role in their pathophysiology. However, the exact molecular mechanisms by which stress hormones directly influence IgE …
BACKGROUND: The majority of bacteria in the vertebrate gut harbor integrated bacterial viruses ("bacteriophages" or "phages"; integrated phage are termed "prophages"). To probe phage replication strategies in the mammalian gut microbiome, we investigated phage activity …
Background: Despite epidemiological interest in aspirin's chemopreventive potential against glioma, the underlying multilayered molecular mechanisms spanning gut microbial ecology, COX-2/PGE2 signaling, inter-organ iron homeostasis via the NEO1/hepcidin regulatory axis, epigenetic reprogramming, and ferroptosis have not …
Endogenous bioelectric currents regulate development and regeneration, but their influence on organismal longevity and stem cell-mediated repair is not well understood. We demonstrate that a brief, clinically safe pulse of electrical current (PEC) produces lasting …
Researchers identified six genetic variants linked to living past 85, 90, and 95 years in a Taiwanese population. When combined with health information, these genetic clues modestly improved predictions of extreme longevity, especially for those …
BACKGROUND: Caloric restriction (CR), achieved by reducing energy intake without malnutrition, has been shown to preserve muscle function and delay age-related declines in strength and mobility by modulating key metabolic and molecular pathways involved in …